EpiTaq HS
Takara EpiTaq HS (for bisulfite-treated DNA) is a DNA polymerase optimized for PCR amplifications using bisulfite-treated DNA containing uracil as template. PCR reactions with bisulfite-treated DNA often pose particular technical challenges for conventional enzymes. TaKaRa EpiTaq HS and the reagents contained in this kit, however, allow adjustment of magnesium and dNTP concentrations to achieve optimal amplification efficiencies and specificities, facilitating excellent amplifications even for targets that previously were impossible to amplify.
Overview
- Superior amplification of bisulfite-treated DNA
- Efficient amplification of a range of amplicons ranging from short (150 bp) to long (1,000 bp)
- EpiTaq HS enzyme inhibits non-specific amplification and primer-dimer formation
- Amplified product can be used directly for TA cloning applications
Applications
- Methylation analysis of CpG Islands
- Amplification of bisulfite-treated DNA, including DNA templates that contain uracils
- DNA amplification of AT/GC rich templates
PCR with bisulfite-treated HeLa genomic DNA as template
PCR with bisulfite-treated HeLa genomic DNA as template.
Components
200 Reactions x 50 µl PCR
| EpiTaq HS 5 U/μl) | 250 U |
| 10X EpiTaq PCR Buffer (Mg2+ free) | 1 ml |
| dNTP Mixture 2.5 mM each dNTP | 1.2 ml |
| 25 mM MgCl2 | 1.2 ml |
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PCR with bisulfite-treated HeLa genomic DNA as template
PCR with bisulfite-treated HeLa genomic DNA as template.
Components
200 Reactions x 50 µl PCR
| EpiTaq HS 5 U/μl) | 250 U |
| 10X EpiTaq PCR Buffer (Mg2+ free) | 1 ml |
| dNTP Mixture 2.5 mM each dNTP | 1.2 ml |
| 25 mM MgCl2 | 1.2 ml |
