EpiScope MSP Kit
EpiScope MSP Kit
Brand: Takara Bio.
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EpiScope MSP Kit
EpiScope MSP Kit contains PCR reagents designed exclusively for methylation-specific PCR (MSP). A specific enzyme combined with an optimized buffer allows MSP analyses using a bisulfite-treated DNA template containing uracil. This kit provides a higher amplification efficiency and greatly improved ability to differentiate methylated/unmethylated DNA compared with traditional PCR reagents. The reaction system has been optimized for real-time monitoring using TB Green, and makes it possible to perform both real-time PCR and endpoint detection under the same reaction conditions.
Overview
- High performance: superior amplification speed and efficiency
- Specific: differentiate methylated/unmethylated DNA by analyzing a single CpG site
- Streamlined: may be used for both real-time (TB Green) and endpoint PCR using the same PCR conditions
Applications
- Methylation-specific PCR for DNA methylation analysis
Components
| 2X MSP Buffer (with Mg2+ and dNTPs)* | 5 x 1 ml |
| MSP Enzyme | 240 µl |
| 100X TB Green | 100 µl |
| ROX Reference Dye (50X conc.) | 200 µl |
| ROX Reference Dye II (50X conc.) | 200 µl |
*The Mg2+ concentration (2X) is 4 mM and the dNTP concentration (2X) is 400 µM.
Storage
Store at –20°C
Note: The 100X TB Green dye is sensitive to light.
Herman, J. G., Graff, J. R., Myohanen, S., Nelkin, B. D. & Baylin, S. B. Methylation-specific PCR: a novel PCR assay for methylation status of CpG islands. Proc. Natl. Acad. Sci. 93, 9821–9826 (1996).
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