Endoproteinase Asp-N
Endoproteinase Asp-N
Brand: Takara Bio.
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Endoproteinase Asp-N
Endoproteinase Asp-N is a metalloprotease which specifically cleaves the peptide bonds at the amino side of aspartic acid or cysteic acid residues of proteins and peptides.
Applications
- Fragmentation of proteins and peptides required for primary structure analysis
Source
Pseudomonas fragi mutant
Purity
Homogeneous on SDS-PAGE. No other proteases detected.
Properties
- Molecular weight: 27 kDa (SDS-PAGE)
- Optimum temperature: 37°C
- Optimum pH: 6.0–8.5
- Inhibitors: 2-phenanthroline, EDTA, DTT
Definition of activity
One azocoll unit is defined as the enzyme activity required to liberate azo dye to an absorbance of 0.001 at 520 nm per minute at 37°C at pH 7.5.
Specific Activity: 20 U/µg or more
Form
Lyophilized (containing the equivalent of 50 µl of 10 mM Tris-HCl, pH 7.5)
Effect of various denaturing agents on enzymatic activityEndoproteinase Asp-N (200 µg/mL) was treated with each denaturant at the indicated concentrationin 25 mM sodium phosphate buffer (pH 7
Effect of various denaturing agents on enzymatic activity
Endoproteinase Asp-N (200 µg/mL) was treated with each denaturant at the indicated concentrationin 25 mM sodium phosphate buffer (pH 7.8). The enzyme was exposed to denaturant for 6 hours at 25°C, then enzyme activity was measured.
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Effect of various denaturing agents on enzymatic activityEndoproteinase Asp-N (200 µg/mL) was treated with each denaturant at the indicated concentrationin 25 mM sodium phosphate buffer (pH 7
Effect of various denaturing agents on enzymatic activity
Endoproteinase Asp-N (200 µg/mL) was treated with each denaturant at the indicated concentrationin 25 mM sodium phosphate buffer (pH 7.8). The enzyme was exposed to denaturant for 6 hours at 25°C, then enzyme activity was measured.
