Enzyme activity is affected by CG methylase depending on the sequence following the recognition site
Star activity: Unrelated sites may often be cut in the presence of a high concentration of glycerol, Mn2+, or DMSO, and at low ionic strength. Enzyme activity reduces to 70 to 80% by the addition of spermine (ca. 0.2 mM), but star activity reduces to 30–50%.
Each lot undergoes stringent quality testing including overdigestion, genome DNA analysis, ligation-recutting and pKF3 cloning
Enzyme activity is affected by CG methylase depending on the sequence following the recognition site
Star activity: Unrelated sites may often be cut in the presence of a high concentration of glycerol, Mn2+, or DMSO, and at low ionic strength. Enzyme activity reduces to 70 to 80% by the addition of spermine (ca. 0.2 mM), but star activity reduces to 30–50%.
Each lot undergoes stringent quality testing including overdigestion, genome DNA analysis, ligation-recutting and pKF3 cloning
