Ligation-recutting test: After digestion with this enzyme, ligation is inefficient due to the structure of the Eco81I fragment ends (Janulaitis, A.A., personal communication). For circularized ligation, the overnight ligation using Takara DNA Ligation Kit, Ver.1 or Ver.2.1 or Mighty Mix is recommended.
Each lot undergoes stringent quality testing including overdigestion, genome DNA analysis, ligation-recutting and pKF3 cloning
Ligation-recutting test: After digestion with this enzyme, ligation is inefficient due to the structure of the Eco81I fragment ends (Janulaitis, A.A., personal communication). For circularized ligation, the overnight ligation using Takara DNA Ligation Kit, Ver.1 or Ver.2.1 or Mighty Mix is recommended.
Each lot undergoes stringent quality testing including overdigestion, genome DNA analysis, ligation-recutting and pKF3 cloning
