This kit provides an easy and efficient method to rescue plasmid DNA from Saccharomyces cerevisiae. The protocol uses Zymolyase for highly efficient enzymatic disruption of yeast cell walls, followed by SDS/alkaline lysis of the resulting spheroplasts, and spin column purification of the plasmid DNA. Purified DNA can then be transformed into E. coli for propagation and purification.
Overview
Rescue plasmids from yeast using spin columns
Highly efficient Zymolyase is included
Purify DNA in less time
Applications
Plasmid DNA minipreps from yeast
Rescue positive prey plasmids after a yeast two-hybrid or yeast one-hybrid screen
The Easy Yeast Plasmid Isolation protocol uses Zymolyase for highly efficient enzymatic disruption of yeast cell walls, followed by SDS/alkaline lysis of the resulting spheroplasts
The Easy Yeast Plasmid Isolation protocol uses Zymolyase for highly efficient enzymatic disruption of yeast cell walls, followed by SDS/alkaline lysis of the resulting spheroplasts. A spin column is used to purify the plasmid DNA, which can then be transformed into E. coli for propagation and scaled-up plasmid preparations, or used as a template for PCR.
This kit provides an easy and efficient method to rescue plasmid DNA from Saccharomyces cerevisiae. The protocol uses Zymolyase for highly efficient enzymatic disruption of yeast cell walls, followed by SDS/alkaline lysis of the resulting spheroplasts, and spin column purification of the plasmid DNA. Purified DNA can then be transformed into E. coli for propagation and purification.
Overview
Rescue plasmids from yeast using spin columns
Highly efficient Zymolyase is included
Purify DNA in less time
Applications
Plasmid DNA minipreps from yeast
Rescue positive prey plasmids after a yeast two-hybrid or yeast one-hybrid screen
The Easy Yeast Plasmid Isolation protocol uses Zymolyase for highly efficient enzymatic disruption of yeast cell walls, followed by SDS/alkaline lysis of the resulting spheroplasts
The Easy Yeast Plasmid Isolation protocol uses Zymolyase for highly efficient enzymatic disruption of yeast cell walls, followed by SDS/alkaline lysis of the resulting spheroplasts. A spin column is used to purify the plasmid DNA, which can then be transformed into E. coli for propagation and scaled-up plasmid preparations, or used as a template for PCR.