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EaeI (CfrI)
EaeI (CfrI) restriction site:
(T/C) | GGCC (A/G)
(A/G) CCGG | (T/C)
Supplied buffer: M
Effect of DNA methylation: enzyme activity is often affected by both dcm and CG methylase
Reaction temperature: 37°C
- Source: Enterobacter aerogenes
- Concentration: 4–12 units/µl
- Substrate for unit definition: lambda DNA
- Each lot undergoes stringent quality testing including overdigestion, genome DNA analysis, ligation-recutting and pKF3 cloning
EaeI (CfrI) restriction site:
(T/C) | GGCC (A/G)
(A/G) CCGG | (T/C)
Supplied buffer: M
Effect of DNA methylation: enzyme activity is often affected by both dcm and CG methylase
Reaction temperature: 37°C
- Source: Enterobacter aerogenes
- Concentration: 4–12 units/µl
- Substrate for unit definition: lambda DNA
- Each lot undergoes stringent quality testing including overdigestion, genome DNA analysis, ligation-recutting and pKF3 cloning
