E. coli-yeast Shuttle Vectors
E. coli-yeast Shuttle Vectors
Brand: Takara Bio.
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E. coli-yeast Shuttle Vectors
E. coli-yeast shuttle vectors
The pAUR vector series includes six E. coli-yeast shuttle vectors, each constructed for a particular application in either Saccharomyces cercevisiae, Schizosaccharomyces, Saccharomyces pombe, or Aspergillis nidulans. These vectors include a novel drug-resistance selective marker that confers Aureobasidin A resistance to transformed yeast or filamentous fungal species.
Overview
- pAUR101 DNA (6,687 bp)—chromosomal integration for S. cerevisiae
- pAUR112 DNA (7,104 bp)
- pAUR123 DNA (6,982 bp)
- pAUR224 DNA (7,638 bp)
- pAUR135 DNA (6,074 bp—vector for marker removal in S. cerevisiae
- pAUR316 DNA (11,663 bp)—autonomously replicating vector for Aspergillis nidulans
Applications
Cloning and transformation in yeast or Aspergillus nidulans systems using Aureobasidin A as a selective antibiotic
Form
10 mM Tris-HCl (pH 8.0), 1 mM EDTA
Concentration
1 µg/µl (concentration for pAUR316 DNA is 0.5 µg/µl)
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