E. coli DNA Ligase
E. coli DNA Ligase
Brand: Takara Bio.
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E. coli DNA Ligase
E. coli DNA Ligase
E. coli DNA Ligase is a ligation enzyme that can be used to join DNA fragments by catalyzing the formation of phosphodiester bonds between double-stranded DNA fragments containing juxtaposed 5'-phosphate termini and 3'-hydroxyl termini in the presence of the NAD cofactor. Both E.coli DNA Ligase and T4 DNA Ligase are used in gene cloning experiments; however, E. coli DNA Ligase can only catalyze ligation of cohesive end-containing DNA fragments via standard reaction conditions.
Applications
- Gene cloning
- Ligation
- Phosphodiester bond formation
Source
Recombinant E. coli
Storage
–20°C
Unit Definition
One unit is defined as the amount of enzyme that ligates >90% of 6 µg of HindIII-restricted DNA fragments in 30 min and 16°C (using a 20-µl reaction volume).
Concentration
60 U/µl
Product citations
Panasenko, S. M., Alazard, R. J. & Lehman, I. R. A simple, three-step procedure for the large scale purification of DNA ligase from a hybrid lambda lysogen constructed in vitro. J. Biol. Chem. 253, 4590–2 (1978).
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