Double-strand specific DNase (ArcticZymes)
Double-strand specific DNase (ArcticZymes)
Brand: ArcticZymes
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Double-strand specific DNase
The double-strand specific DNase from Arctic Shrimp (Pandalus borealis) is from a previously unknown gene family. It displays a unique double-strand specificity allowing it to perform in the presence of single stranded DNA molecules such as primers and probes. Ideal for protocols that allows for a discrete heat-inactivation step.
Main advantages with dsDNase
- Double-strand DNA specific endonuclease
- High specific activity
- Can be heat-inactivated by moderate heat treatment (65°C for 15 minutes)
- Producing 5′-phospho-oligonucleotide products
Heat inactivation
The dsDNase can be heat inactivated by heat treatment at 15 min at 65˚C, or 20 min at 60˚C. The enzyme requires 1 mM DTT and pH ≥ 8 for complete inactivation.
Specifications
- Unit definition
One Unit is defined as an increase in absorbance at 260 nm of 0.001 per minute, using 50 µg/ml high MW DNA in 100 mM Na-acetate pH 5.0 and 5 mM MgCl2. - Specific activity
Ca. 400 000 Kunitz Units/mg. - Activity
The dsDNase is highly active in a temperature range of 20-40°C. It needs at least 2.5 mM Mg for activity and has an optimal pH at 7.5. - Storage buffer
20 mM Tris-HCl pH 7.5, 2 mM MgCl2, 10 mM NaCl, 0.01% (v/v) Triton X-100, 50% (v/v) glycerol. - Purity
dsDNase is purified to apparent homogeneity. - Storage
Minimum shelf life is 3 years at -20°C. Storage at 4°C is possible for at least 6 months. The enzyme also tolerates multiple freeze-thaw cycles.
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