DNA Polymerase I, Klenow Fragment
DNA Polymerase I, Klenow Fragment
Brand: Takara Bio.
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DNA Polymerase I, Klenow Fragment
DNA Polymerase I, Klenow Fragment
DNA Polymerase I, Klenow Fragment (Large Fragment) is a proteolytic product of E. coli DNA Polymerase I, which possesses the 5'→3' polymerase and 3'→5' exonuclease activities of intact DNA Polymerase I, but lacks the 5'→3' exonuclease activity of full-length DNA Polymerase I. The lack of 5'→3' exonuclease activity enables the DNA Polymerase I, Klenow Fragment to be used in random primer labeling and DNA sequencing experiments in addition to second-strand cDNA synthesis. The enzyme is supplied in a buffer of 50 mM potassium phosphate (pH 6.5), 1 mmDTT and 50% glyerol (2140A/B) or ethylene glycol (2140AK/BK).
Applications
- Random primer labeling
- Second-strand cDNA synthesis
- Dideoxy DNA sequencing
- 2140AK/BK are sequencing grade reagents
Buffer
Supplied with 10X Reaction Buffer [100 mM Tris-HCl (pH 7.5), 70 mM MgCl2 and 1 mM DTT].
Concentration
- 2140A/B: 2–5 U/µl
- 2140AK/BK: 2 U/µl
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