DNA Methylation Analysis: MethylEasy Xceed
DNA Methylation Analysis: MethylEasy Xceed
Brand: Takara Bio.
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DNA Methylation Analysis: MethylEasy Xceed
MethylEasy Xceed is a simple, sensitive and quick bisulphite conversion treatment. Requires no DNA pre-treatment and uses as little as 50 pg of starting material. Results in virtually no DNA loss and increased DNA template stability.
Overview
- Easy to use: no DNA pretreatment necessary; easy column purification
- More conversions per kit: sufficient reagent for up to 40 conversion reactions
- Quicker reaction time: processing time is 90 min from wild type to fully modified DNA
- High sensitivity: start with as little as 50 pg of DNA (~8 mammalian cell equivalents)
- Excellent DNA preservation: reduced DNA degradation (>90%) for true analysis of methylated cytosine
- Improved DNA conversion: no evidence of nonconversion found in sequence analysis
- Internal controls are included for experimental outcome confidence and troubleshooting
Applications
- Detection and quantification of transgenes inserted into transgenic mouse genome
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