DNA Ligation Kit, Quick
DNA Ligation Kit, Quick
Brand: Takara Bio.
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DNA Ligation Kit, Quick
DNA ligation kits, quick
DNA Ligation Kit, Version 1 and DNA Ligation Kit, Version 2.1 enable high-efficiency ligation of DNA molecules in vitro. Using an optimized buffer system and T4 DNA Ligase, these DNA ligation kits offer fast ligation in 30 minutes. Ligation reactions can be used directly in selected bacterial transformations with no need for DNA purification.
DNA Ligation Kit, Version 1 and DNA Ligation Kit, Version 2.1 provide the fliexibility of formats, with the DNA Ligation Kit, Version 2.1 containing a convenient premix of enzyme and buffer that further minimizes pipetting steps and permits small final reaction volumes (for standard reactions). By using Solution II of the DNA Ligation Kit, Version 2.1, linker ligation may also be performed. Transformation efficiency can be further improved by the addition of Solution III (Transformation Enhancer) into circular ligation reactions prior to transformation. The DNA Ligation Kit, Version 1.0 can be used for DNA concatenation via the addition of Solution B alone.
Applications
- High-efficiency ligation in 3 minutes (30 minutes for most applications)
- Quick ligation for cloning in 3 minutes (30 minutes for some applications)
- DNA insertion into lambda phage vector (Ver. 1)
- Small-volume ligation (Ver. 2.1)
Fast ligation of a DNA fragment into a phage vector
Fast ligation of a DNA fragment into a phage vector. Control insert DNA (plasmid vector) digested with EcoR I was ligated into dephosphorylated lambda gt11/EcoR I arms for 3 minutes at 25°C.
Efficiency of Linker Ligation
Efficiency of Linker Ligation. A pBgl II linker d[pCAGAATCTG] was ligated with dephosphorylated Hinc II pUC118 DNA. Ten nanograms of the ligation reaction was transformed into E. coli JM109 Competent Cells (1.3 x 108 transformants/µg DNA), and the transformation efficiency was calculated from the yield of white colonies.
DNA Ligation Kit recommended reaction conditions
DNA Ligation Kit recommended reaction conditions.
Product citation
Hayashi, K. et al., Regulation of inter- and intramolecular ligation with T4 DNA ligase in the presence of polyethylene glycol. Nucleic Acids Res. 14, 7617–7631 (1986).
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Fast ligation of a DNA fragment into a phage vector
Fast ligation of a DNA fragment into a phage vector. Control insert DNA (plasmid vector) digested with EcoR I was ligated into dephosphorylated lambda gt11/EcoR I arms for 3 minutes at 25°C.
Efficiency of Linker Ligation
Efficiency of Linker Ligation. A pBgl II linker d[pCAGAATCTG] was ligated with dephosphorylated Hinc II pUC118 DNA. Ten nanograms of the ligation reaction was transformed into E. coli JM109 Competent Cells (1.3 x 108 transformants/µg DNA), and the transformation efficiency was calculated from the yield of white colonies.
DNA Ligation Kit recommended reaction conditions
DNA Ligation Kit recommended reaction conditions.
