DNA Ligation Kit, Mighty Mix
DNA Ligation Kit, Mighty Mix
Brand: Takara Bio.
In stock
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DNA Ligation Kit, Mighty Mix
DNA Ligation Kit, Mighty Mix
Takara's DNA Ligation Kit, Mighty Mix, is a one-solution premix ligation reagent that offers high efficiency ligations, particularly for blunt-ended ligation. The reaction can be performed for 5 minutes at 25°C (rapid protocol), or 30 minutes at 16°C (standard protocol) with equal results for both circular and linear DNA ligations. The 2X Ligation Mix allows small ligation reaction volumes (10 μl) when DNA is limiting. The kit includes sufficient reagent for 100 ligation reactions. Ligation products reaction can be used directly in bacterial transformation.
Overview
- Enables high-efficiency ligations for cohesive, blunt-end, and TA cloning
- 2X ligation mix adds convenience and eliminates extra pipetting and purification steps
- Ligation reactions can be quickly performed at either 5 or 30 minutes depending on DNA type
- Reaction volumes are scalable
Applications
- Highly efficient and fast DNA ligation
- Small reaction volume ligation
- TA and blunt-end cloning
Blunt-end ligation efficiency with Takara-brand DNA Ligation Kit, Mighty Mix and similar kits from three competitors
Blunt-end ligation efficiency with Takara-brand DNA Ligation Kit, Mighty Mix and similar kits from three competitors. Each ligation reaction contained 25 fmol of BAP-treated pUC118-Hinc II vector and 75 fmol of a 500 bp insert. Ligation efficiency was assessed by blue/white colony screening.
TA-vector ligation efficiency of Takara-brand DNA Ligation Kit, Mighty Mix and similar kits from three competitors
TA-vector ligation efficiency of Takara-brand DNA Ligation Kit, Mighty Mix and similar kits from three competitors. Each ligation reaction contained 13 fmol of a pT7 Blue T-Vector and 39 fmol of a 2.08 kb PCR Product. Ligation efficiency was assessed by blue/white colony screening.
Recommended reaction conditions
Recommended reaction conditions.
Note: This kit retains activity through 50 freeze-thaw cycles. The Ligation Mix should be thawed on ice (5-10 minutes) and mixed well by pipetting prior to use.
Product citation
Hayashi, K., Nakazawa, M., Ishizaki, Y., Hiraoka, N. & Obayashi, A. Regulation of inter- and intramolecular ligation with T4 DNA ligase in the presence of polyethylene glycol. Nucleic Acids Res. 14, 7617–31 (1986).
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Blunt-end ligation efficiency with Takara-brand DNA Ligation Kit, Mighty Mix and similar kits from three competitors
Blunt-end ligation efficiency with Takara-brand DNA Ligation Kit, Mighty Mix and similar kits from three competitors. Each ligation reaction contained 25 fmol of BAP-treated pUC118-Hinc II vector and 75 fmol of a 500 bp insert. Ligation efficiency was assessed by blue/white colony screening.
TA-vector ligation efficiency of Takara-brand DNA Ligation Kit, Mighty Mix and similar kits from three competitors
TA-vector ligation efficiency of Takara-brand DNA Ligation Kit, Mighty Mix and similar kits from three competitors. Each ligation reaction contained 13 fmol of a pT7 Blue T-Vector and 39 fmol of a 2.08 kb PCR Product. Ligation efficiency was assessed by blue/white colony screening.
Recommended reaction conditions
Recommended reaction conditions.
Note: This kit retains activity through 50 freeze-thaw cycles. The Ligation Mix should be thawed on ice (5-10 minutes) and mixed well by pipetting prior to use.
