DNA Fragmentation Kit
DNA Fragmentation Kit
Brand: Takara Bio.
In stock
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DNA Fragmentation Kit
This kit is designed to perform random fragmentation of genomic DNAs and other long-chain dsDNAs by enzyme treatments without any special apparatus such as a sonicator, and then to blunt the obtained DNA fragment. Blunt-end fragments may be inserted into blunt-end vectors. The reaction may be stopped after fragmentation is complete if no blunting is required. This kit may also be used to perform the pretreatment in methylated DNA enrichment and high-speed DNA sequence analysis.
Applications
- Genomic DNA fragmentation
- dsDNA fragmentation
Components
| 20 preparations | |
|---|---|
| Enzyme-1 | 20 µl |
| Dilution Buffer-1 | 1,040 µl |
| A Solution | 20 µl |
| B Solution | 50 µl |
| Stop Solution | 400 µl |
| 150 mM MgCl2 | 40 µl |
| Dilution Buffer-2 | 200 µl |
| Enzyme-2 | 20 µl |
| 0.5 M EDTA | 50 µl |
| dH2O | 10 × 1 ml |
Storage
–20°C. Solution A, Solution B, 150 mM MgCl2, 0.5 M EDTA and dH2O may be stored at 4°C.
Materials required but not provided
- Thermal cycler (at least 1 cycler is required; 2 cyclers are preferred)
- Electrophoresis loading buffer
We recommend using a loading buffer that contains a dye (e.g., Orange G) that does not overlap with dsDNA fragmentation bands ranging from 100 bp to 1,000 bp in size on the electrophoresis gel. When using BPB or xylene cyanol, please be aware that these dyes overlap with dsDNA fragments in the 100 bp to 1,000 bp size range.
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