DNA Blunting Kit
DNA Blunting Kit
Brand: Takara Bio.
In stock
SKU
DNA Blunting Kit
DNA Blunting Kit
The DNA Blunting Kit allows the conversion of 3' and 5' overhangs to blunt ends. This conversion is accomplished by the 3'→5' exonuclease and 5'→3' polymerase activities of T4 DNA Polymerase. The resulting blunt-ended DNA can be ligated efficiently into a vector using the same optimized buffer system used in Takara's DNA Ligation Kits, then used directly in bacterial transformation or in vitro packaging procedures without DNA purification.
Applications
- Quick and easy blunting of DNA fragment ends
Product citations
Sambrook, J., Fritsch, E. F. & Maniatis, T. in Mol. Cloning A Lab. Manual. Cold Spring Harb. Lab. Press 931–957 (Cold Spring Harbor Laboratory Press, 1989).
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