DmrC Polyclonal Antibody
DmrC Polyclonal Antibody
Brand: Takara Bio.
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DmrC Polyclonal Antibody
The DmrC Polyclonal Antibody was raised in rabbit and recognizes as little as 20–40 ng of DmrC-tagged fusion protein expressed using any iDimerize Inducible Heterodimer System. The antibodies are also able to detect fusions created using ARGENT regulation systems: DmrA is identical to wild type FKBP12, DmrB is identical to the F36V mutant of FKBP12, DmrC is identical to the T82L mutant of FRB.
Overview
- The DmrB and DmrA domains are highly homologous, so the antibodies cross-react with each others' domains
- The anti-DmrC antibody does not cross-react with the other domains
- Detection of DmrB domains used for iDimerize Homodimer Systems
- Detection of DmrA and DmrC domains used for iDimerize Heterodimer Systems
- DmrB antibody can be used for detection of DmrD domains used for the Reverse Dimerization Systems
- DmrA is identical to wild type FKBP12
- DmrB is identical to the F36V mutant of FKBP12
- DmrC is identical to the T82L mutant of FRB
Source
- DmrA and DmrB monoclonal antibodies: mouse
- DmrC polyclonal antibody: rabbit
Western blot detection of a DmrC-tagged protein with the DmrC Polyclonal Antibody (Cat. No. 635091)
Western blot detection of a DmrC-tagged protein with the DmrC Polyclonal Antibody (Cat. No. 635091). HeLa cells were transfected with a plasmid engineered to express a DmrC-AcGFP1 fusion protein. Lysate was prepared from the equivalent of 3.5 x 104 cells and resolved on a polyacrylamide gel, followed by Western blot analysis with the DmrC Polyclonal Antibody (1:1000 dilution). A single band was detected at the expected molecular weight (Lane 3). No band was detected in lysate from untransfected HeLa cells (Lane 1) or HEK 293 cells (Lane 2).
The DmrC Polyclonal Antibody (Cat. No. 635091) can detect as little as 16 ng of DmrC protein
The DmrC Polyclonal Antibody (Cat. No. 635091) can detect as little as 16 ng of DmrC protein. The indicated amounts of recombinant DmrC protein (12 kDa) were resolved by SDS-PAGE and subjected to Western blot analysis. The DmrC Polyclonal Antibody was able to detect less than 16 ng of purified protein. Lysate from HEK 293 cells was used as a negative control (Lane 1).
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Western blot detection of a DmrC-tagged protein with the DmrC Polyclonal Antibody (Cat. No. 635091)
Western blot detection of a DmrC-tagged protein with the DmrC Polyclonal Antibody (Cat. No. 635091). HeLa cells were transfected with a plasmid engineered to express a DmrC-AcGFP1 fusion protein. Lysate was prepared from the equivalent of 3.5 x 104 cells and resolved on a polyacrylamide gel, followed by Western blot analysis with the DmrC Polyclonal Antibody (1:1000 dilution). A single band was detected at the expected molecular weight (Lane 3). No band was detected in lysate from untransfected HeLa cells (Lane 1) or HEK 293 cells (Lane 2).
The DmrC Polyclonal Antibody (Cat. No. 635091) can detect as little as 16 ng of DmrC protein
The DmrC Polyclonal Antibody (Cat. No. 635091) can detect as little as 16 ng of DmrC protein. The indicated amounts of recombinant DmrC protein (12 kDa) were resolved by SDS-PAGE and subjected to Western blot analysis. The DmrC Polyclonal Antibody was able to detect less than 16 ng of purified protein. Lysate from HEK 293 cells was used as a negative control (Lane 1).
