DmrB Monoclonal Antibody
DmrB Monoclonal Antibody
Brand: Takara Bio.
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DmrB Monoclonal Antibody
The DmrB Monoclonal Antibody recognizes DmrB-tagged fusion proteins expressed using any iDimerize Inducible Homodimer System. The isotype of this mouse monoclonal antibody is IgG1 Kappa. The antibodies are also able to detect fusions created using ARGENT regulation systems: DmrA is identical to wild type FKBP12, DmrB is identical to the F36V mutant of FKBP12, DmrC is identical to the T82L mutant of FRB.
Overview
- The DmrB and DmrA domains are highly homologous, so the antibodies cross-react with each others' domains
- The anti-DmrC antibody does not cross-react with the other domains
- Detection of DmrB domains used for iDimerize Homodimer Systems
- Detection of DmrA and DmrC domains used for iDimerize Heterodimer Systems
- DmrB antibody can be used for detection of DmrD domains used for the Reverse Dimerization Systems
- DmrA is identical to wild type FKBP12
- DmrB is identical to the F36V mutant of FKBP12
- DmrC is identical to the T82L mutant of FRB
Source
- DmrA and DmrB monoclonal antibodies: mouse
- DmrC polyclonal antibody: rabbit
Western blot detection of a DmrB-tagged protein using the DmrB Monoclonal Antibody (Cat.No. 635090)
Western blot detection of a DmrB-tagged protein using the DmrB Monoclonal Antibody (Cat. # 635090). HEK 293 cells were transfected with the pAcGFP1-DmrB plasmid. Lysate from the equivalent of 3.5 x 104 cells was run on a polyacrylamide gel (Lanes 2 and 3) and analyzed by Western blot using the DmrB Monoclonal Antibody (1:1000 dilution). A predominant band was detected at the expected molecular weight. The band was detected in lysate from cells treated with (Lane 3) or without (Lane 2) B/B Homodimerizer, but not in lysate from untransfected HEK 293 cells (Lane 1).
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Western blot detection of a DmrB-tagged protein using the DmrB Monoclonal Antibody (Cat.No. 635090)
Western blot detection of a DmrB-tagged protein using the DmrB Monoclonal Antibody (Cat. # 635090). HEK 293 cells were transfected with the pAcGFP1-DmrB plasmid. Lysate from the equivalent of 3.5 x 104 cells was run on a polyacrylamide gel (Lanes 2 and 3) and analyzed by Western blot using the DmrB Monoclonal Antibody (1:1000 dilution). A predominant band was detected at the expected molecular weight. The band was detected in lysate from cells treated with (Lane 3) or without (Lane 2) B/B Homodimerizer, but not in lysate from untransfected HEK 293 cells (Lane 1).
