CellAmp Direct RNA Prep Kit for RT-PCR
CellAmp Direct RNA Prep Kit for RT-PCR
Brand: Takara Bio.
In stock
SKU
CellAmp Direct RNA Prep Kit for RT-PCR
CellAmp direct RNA prep kit for real-time PCR
The CellAmp Direct RNA Prep Kit for RT-PCR (qPCR) is for preparation of templates used in one- and two-step real-time RT-PCR, utilizing a simple method of extraction directly from cultured animal cells in 96 well-plates or other vessels. It is possible to prepare templates for real-time PCR from cultured animal cells in approximately 10 minutes.
Using this kit along with a one-step real-time RT-PCR kit (such as the One Step TB Green Ex Taq qRT-PCR kit) allows gene expression analysis to be performed in approximately two hours. Templates can be prepared directly from small quantities of animal cells, which is useful for analysis of a gene expression profile and does not influence the high sensitivity of real-time RT-PCR. Genomic DNA can be removed efficiently using this kit, making it a powerful tool for expression analysis of genes for which primers can't be designed on both ends an exon junction, or genes expressed at low levels.
Overview
- Quick (10 minutes) and simple preparation of RNA template from cultured animal cells
- RNA can be used for one- or two-step qRT-PCR
- Works with intercalating green dye or probe detection
- Gene analysis in two hours
- Easy, time-saving, and flexible prep kit
Applications
- Prepare RNA templates for qRT-PCR
Write Your Own Review
