CellAmp Direct RNA Prep Kit for qPCR or Protein Analysis
CellAmp Direct RNA Prep Kit for qPCR or Protein Analysis
Brand: Takara Bio.
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CellAmp Direct RNA Prep Kit for qPCR or Protein Analysis
Straightforward RNA isolation: CellAmp direct RNA prep kit for qPCR or protein analysis
The CellAmp Direct RNA Prep Kit for qRT-PCR and Protein Analysis allows simple, direct extraction directly from cultured cells to prepare templates used for one-step and two-step qRT-PCR. The kit can be used for cells cultured in 96-well plates and is compatible with high-throughput studies. Preparation of templates for real-time PCR from cultured cells takes approximately 10 minutes and complete gene expression analysis (via One Step or Two Step qRT-PCR) is performed in approximately 2 hours. When used in combination with a reverse transcription kit, cDNA templates for real-time PCR can be synthesized in about 30 minutes, providing efficient high-throughput analysis. Templates can be prepared directly from small quantities of cells for analysis by high sensitivity qRT-PCR. Genomic DNA can be easily and efficiently removed using this kit. Excellent for detection of rare transcripts.
Overview
- Easy RNA isolation protocol—prepare template from cultured animal cells in only 10 minutes
- Prepared RNA can be used for one- or two-step qRT-PCR
- Works with green intercalating dye assays and probe assays
- Gene expression analysis can be completed in ~2 hours
Applications
- RNA template preparation from cultured animal cells for qPCR
Product citations
Kato, Y. et al. A lentiviral vector encoding two fluorescent proteins enables imaging of adenoviral infection via adenovirus-encoded miRNAs in single living cells. J. Biochem. 147:63–71 (2010).
Nemoto, T. et al. Role of urocortin 2 secreted by the pituitary in the stress-induced suppression of luteinizing hormone secretion in rats. Am. J. Physiol. Endocrinol. Metab. 299:E567–E575 (2010).
Nishitsuji, K. et al. Apolipoprotein E regulates the integrity of tight junctions in an isoform-dependent manner in an in vitro blood-brain barrier model. J. Biol. Chem. 286:17536–17542 (2011).
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