Cellartis Definitive Endoderm Differentiation Kit
Cellartis Definitive Endoderm Differentiation Kit
Brand: Takara Bio.
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Cellartis Definitive Endoderm Differentiation Kit
The Cellartis Definitive Endoderm Differentiation Kit contains complete media and a ready-to-use coating for the differentiation of human induced pluripotent stem (iPS) cells to definitive endoderm (DE) cells in 2D culture. The complete Cellartis Definitive Endoderm Differentiation Kit with DEF-CS Culture System contains the Cellartis Definitive Endoderm Differentiation Kit plus the Cellartis DEF-CS 100 Culture System, which is used to culture undifferentiated human iPS cells.
Overview
- Efficient differentiation—generates >80% SOX17-positive cells
- Robust protocol—successful definitive endoderm (DE) cell generation from more than 25 different hiPS cell lines
- Complete kit—media and ready-to-use coating for a seven-day protocol to reproducibly differentiate hiPS cells into DE cells
- Start with a homogeneous population of hiPS cells—kit includes the Cellartis DEF-CS 100 Culture System to maintain undifferentiated cells prior to the start of DE cell differentiation
mRNA expression profiles of definitive endoderm cells being generated from two hiPS cell lines (ChiPSC18 and P11025) using the Cellartis Definitive Endoderm Differentiation Kit with DEF-CS Culture Medium
mRNA expression profiles of definitive endoderm cells being generated from two hiPS cell lines (ChiPSC18 and P11025) using the Cellartis Definitive Endoderm Differentiation Kit with DEF-CS Culture Medium. These graphs track the relative temporal mRNA expression levels of various markers during differentiation from hiPS cells (starting on Day 0) into DE cells (by Day 7), as measured by RT-qPCR.
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mRNA expression profiles of definitive endoderm cells being generated from two hiPS cell lines (ChiPSC18 and P11025) using the Cellartis Definitive Endoderm Differentiation Kit with DEF-CS Culture Medium
mRNA expression profiles of definitive endoderm cells being generated from two hiPS cell lines (ChiPSC18 and P11025) using the Cellartis Definitive Endoderm Differentiation Kit with DEF-CS Culture Medium. These graphs track the relative temporal mRNA expression levels of various markers during differentiation from hiPS cells (starting on Day 0) into DE cells (by Day 7), as measured by RT-qPCR.
