The ProteoTuner DD Monoclonal Antibody recognizes the DD-N and DD-C domains, which have been optimized for use as N- and C-terminal fusion tags respectively.
Easily detect ProteoTuner shield system DD fusions with the DD Monoclonal Antibody
Easily detect ProteoTuner shield system DD fusions with the DD Monoclonal Antibody. Cell lysates from HeLa cells transiently expressing either DD-AcGFP1 or AcGFP1-DD and HEK 293 cells stably expressing DD-AcGFP1 were analyzed by Western blot using the DD Monoclonal Antibody at a 1:500 dilution. Lane 1: HeLa cells transfected with pDD-AcGFP1 (e.g., DD-N). Lane 2: Negative control (untransfected HeLa cells). Lane 3: HeLa cells transfected with pAcGFP1-DD (e.g., DD-C). Lane 4: Negative control (untransfected HEK 293 cells). Lane 5: HEK 293 cells stably expressing DD-AcGFP1.
The ProteoTuner DD Monoclonal Antibody recognizes the DD-N and DD-C domains, which have been optimized for use as N- and C-terminal fusion tags respectively.
Easily detect ProteoTuner shield system DD fusions with the DD Monoclonal Antibody
Easily detect ProteoTuner shield system DD fusions with the DD Monoclonal Antibody. Cell lysates from HeLa cells transiently expressing either DD-AcGFP1 or AcGFP1-DD and HEK 293 cells stably expressing DD-AcGFP1 were analyzed by Western blot using the DD Monoclonal Antibody at a 1:500 dilution. Lane 1: HeLa cells transfected with pDD-AcGFP1 (e.g., DD-N). Lane 2: Negative control (untransfected HeLa cells). Lane 3: HeLa cells transfected with pAcGFP1-DD (e.g., DD-C). Lane 4: Negative control (untransfected HEK 293 cells). Lane 5: HEK 293 cells stably expressing DD-AcGFP1.