CultiLife 215 Culture Bag

The CultiLife 215 Culture Bag (Cat. # FU0005) is composed of polyethylene and can be coated with RetroNectin reagent for high-efficiency retroviral or lentiviral transduction of hematopoietic cells. After transduction the cells can then be expanded into the larger GT-T610 (CultiLife Eva) bag (Cat. # FU0010), which is composed of ethylene vinyl acetate (EVA). Note: only the CultiLife 215 Culture Bag can be coated with RetroNectin reagent. CultiLife 215 cell culture bags are designed for medium to large-scale cell culture. Each bag has a culture area of 215 cm2 and has a maximum volume capacity of 300 ml.

Overview

For more specifications and an example protocol, visit the CultiLife culture bags protocol page.
Sterile, closed-system cell culture in a single bag
The CultiLife 215 Culture Bag is compatible with RetroNectin reagent for transduction experiments
CultiLife 215 has a culture area of 215 cm²
Improved cell proliferation allows for greater transduction efficiency
Transparent surface allows microscopic observation
Reduces risk of culture contamination
Use less space than traditional flasks

Applications

Sterile cell culture
Gene transfer by lentiviral or retroviral transduction

The CultiLife 215 Culture Bag has 215 cm2 of culture area

The CultiLife 215 Culture Bag has 215 cm² of culture area.

The filled Cultilife cell-culture bag easily fits in an incubator

The filled Cultilife cell-culture bag easily fits in an incubator. The GT-T610 (CultiLife Eva) Culture Bag is shown.

Product citations

Chono, H. et al. In Vivo Safety and Persistence of Endoribonuclease Gene-Transduced CD4+ T Cells in Cynomolgus Macaques for HIV-1 Gene Therapy Model. PLoS One 6, e23585 (2011).

Yu, S. S. et al. In vivo persistence of genetically modified T cells generated ex vivo using the fibronectin CH296 stimulation method. Cancer Gene Ther. 15, 508–516 (2008).