CREB Dominant-Negative Vector Set
CREB Dominant-Negative Vector Set
Brand: Takara Bio.
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CREB Dominant-Negative Vector Set
Set of three vectors for studying CREB-related signaling pathways. The pCMV-CREB Vector expresses the wild-type CREB protein. pCMVCREB133 Vector encodes a mutated form of CREB, blocking cAMP-induced transcription and preventing CREB phosphorylation. pCMVKCREB also encodes a mutated form of CREB, blocking cAMP-induced transcription. Each gene is expressed from the constitutive CMV promoter.
Overview
- Simplify the complexities of signal transduction in vivo
- Dissect the network of signal transduction pathways
- Screen for compounds that activate key pathways
- Combine with cis reporter vectors to create a complete assay system
Signal transduction kits are available for in vivo studies of signal transduction—from signaling cascades to gene transcription
Signal transduction kits are available for in vivo studies of signal transduction—from signaling cascades to gene transcription.
Inhibition of NFκB activity using pCMV-IκBα and pCMVκIαBM from the IκB Dominant-Negative Vector Set
Inhibition of NFκB activity using pCMV-IκBα and pCMVκIαBM from the IκB Dominant-Negative Vector Set (Cat. # 631923). HEK 293 cells were transiently transfected with the indicated plasmids. After 16 hr, fresh medium was added to the cells along with TNF to induce the NFκB pathway. SEAP activity was assayed 6 hr later to determine NFκB activity.
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Signal transduction kits are available for in vivo studies of signal transduction—from signaling cascades to gene transcription
Signal transduction kits are available for in vivo studies of signal transduction—from signaling cascades to gene transcription.
Inhibition of NFκB activity using pCMV-IκBα and pCMVκIαBM from the IκB Dominant-Negative Vector Set
Inhibition of NFκB activity using pCMV-IκBα and pCMVκIαBM from the IκB Dominant-Negative Vector Set (Cat. # 631923). HEK 293 cells were transiently transfected with the indicated plasmids. After 16 hr, fresh medium was added to the cells along with TNF to induce the NFκB pathway. SEAP activity was assayed 6 hr later to determine NFκB activity.
