CloneAmp HiFi PCR Premix
CloneAmp HiFi PCR Premix
Brand: Takara Bio.
In stock
SKU
CloneAmp HiFi PCR Premix
CloneAmp HiFi PCR Premix—high-fidelity PCR for cloning
CloneAmp HiFi PCR Premix is designed for use with the In-Fusion Cloning system due to its exceptionally accurate and efficient DNA amplification. The 2X master mix contains enzyme, optimized buffer, and dNTPs, allowing rapid setup of PCR reactions and facilitating high-throughput applications for multiple cloning samples.
Overview
- Highly accurate—exceptionally low error rate (12 mismatched bases per 542,580 total bases)
- Fast—quick priming and extension (5 sec/kb) enable fast PCR reactions
- Specific—hot-start antibody prevents nonspecific amplification
- Versatile—works with long (up to 10 kb) and GC-rich templates
- Convenient—premix allows rapid reaction setup
Mutation frequency of CloneAmp HiFi Polymerase compared to other high-fidelity PCR enzymes
Mutation frequency of CloneAmp HiFi Polymerase compared to other high-fidelity PCR enzymes. Eight arbitrarily selected GC-rich regions were amplified with CloneAmp HiFi Polymerase or other DNA polymerases using a Thermus thermophilus HB8 genomic DNA template, and cloned into suitable plasmids. Multiple clones were selected for each amplification product and subjected to sequence analysis. DNA fragments amplified using CloneAmp HiFi Polymerase yielded only 12 mismatched bases per 542,580 total bases—lower than an alternative high-fidelity enzyme from Company A, and 10-fold lower than Taq DNA polymerase.
Write Your Own Review
Mutation frequency of CloneAmp HiFi Polymerase compared to other high-fidelity PCR enzymes
Mutation frequency of CloneAmp HiFi Polymerase compared to other high-fidelity PCR enzymes. Eight arbitrarily selected GC-rich regions were amplified with CloneAmp HiFi Polymerase or other DNA polymerases using a Thermus thermophilus HB8 genomic DNA template, and cloned into suitable plasmids. Multiple clones were selected for each amplification product and subjected to sequence analysis. DNA fragments amplified using CloneAmp HiFi Polymerase yielded only 12 mismatched bases per 542,580 total bases—lower than an alternative high-fidelity enzyme from Company A, and 10-fold lower than Taq DNA polymerase.
