CLEARLY Stained Protein Ladder is a mixture of 11 pre-stained recombinant highly purified proteins. When this ladder is analyzed by SDS-PAGE, it is resolved into clearly identifiable visible bands from 10 to 210 kDa. The 30 and 70 kDa bands have quadruple the mass of the other bands and serve as reference indicators. This ladder is pre-mixed with loading buffer and ready to load. Pre-heating is not required. 10 μl of this ladder is loaded per lane of SDS-PAGE minigel for standard use. In this case, this ladder is sufficient for 50 lanes. This ladder is useful for monitoring of protein migration during SDS-PAGE and verifying transfer efficiency at western blotting.＊

＊: Because the covalent coupling of the dye to the proteins affects their electrophoretic behavior on SDS-PAGE gels relative to unstained proteins, this ladder is not recommended for use as a precise size standard when performing SDS-PAGE to calculate the molecular weight of a protein of interest.

SDS-PAGE recommendations

• 5–20% gradient gels are recommended.
• Unstained ladder (Cat.# 3453): Load 5 µl per lane.
• Pre-stained ladder (Cat.# 3454): Load 10 µl per lane.
• Ensure that the ladder is completely thawed before removing an aliquot for loading.

Storage

–20°C

These products are stable for 6 months when stored at 4°C. They remain stable for up to 50 freeze-thaw cycles.

Quantity

CLEARLY Protein Ladder (Unstained) is sufficient for 100 lanes. CLEARLY Stained Protein Ladder is sufficient for 50 lanes.