CLEARLY Protein Ladder (Unstained) is a mixture of 11 recombinant highly purified proteins. When this ladder is analyzed by SDS-PAGE and stained with Coomassie Brilliant Blue R-250, it is resolved into quite clearly identifiable bands from 10 to 250 kDa. This ladder is intended for use as a precise size standard when performing SDS-PAGE to calculate the molecular weight of a protein of interest. The 30 and 80 kDa bands have quadruple the mass of the other bands and serve as reference indicators. This ladder is pre-mixed with loading buffer and ready to load. Preheating is not required. 5 μl of this ladder is loaded per lane of SDS-PAGE minigel for standard use. In this case, this ladder is sufficient for 100 lanes.

SDS-PAGE recommendations

• 5–20% gradient gels are recommended.
• Unstained ladder (Cat.# 3453): Load 5 µl per lane.
• Pre-stained ladder (Cat.# 3454): Load 10 µl per lane.
• Ensure that the ladder is completely thawed before removing an aliquot for loading.

Storage

–20°C

These products are stable for 6 months when stored at 4°C. They remain stable for up to 50 freeze-thaw cycles.

Quantity

CLEARLY Protein Ladder (Unstained) is sufficient for 100 lanes. CLEARLY Stained Protein Ladder is sufficient for 50 lanes.