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ClaI
ClaI restriction site:
AT | CGAT
TAGC | TA
Supplied buffer: M
Effect of DNA methylation: enzyme activity is often affected by both CG and dam methylase
Reaction temperature: 30°C
- Source: Caryophanon latum L
- Concentration: 10 units/µl [High Concentration: 50 units/µl]
- Substrate for unit definition: lambda DNA
- Genomic DNA analysis: Arthrobacter luteus genome DNA
- Note: this enzyme shows equivalent activity at 37°C compared to 30°C
- Each lot undergoes stringent quality testing including overdigestion, genome DNA analysis, ligation-recutting and pKF3 cloning
ClaI restriction site:
AT | CGAT
TAGC | TA
Supplied buffer: M
Effect of DNA methylation: enzyme activity is often affected by both CG and dam methylase
Reaction temperature: 30°C
- Source: Caryophanon latum L
- Concentration: 10 units/µl [High Concentration: 50 units/µl]
- Substrate for unit definition: lambda DNA
- Genomic DNA analysis: Arthrobacter luteus genome DNA
- Note: this enzyme shows equivalent activity at 37°C compared to 30°C
- Each lot undergoes stringent quality testing including overdigestion, genome DNA analysis, ligation-recutting and pKF3 cloning
