Chloramphenicol-resistant pUC Vectors
Chloramphenicol-resistant pUC Vectors
Brand: Takara Bio.
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Chloramphenicol-resistant pUC Vectors
Chloramphenicol-resistant pUC vectors
The chloramphenicol-resistant pUC vectors pHSG396 and pHSG398 contain the chloramphenicol resistance gene, the pMB1-derived origin of replication (ori), and the beta-galactosidase coding gene lacZ. These vectors also contain a pUC18-derived multiple cloning site (MCS) within the lacZ gene, enabling recombinant clones to be verified through culture plates containing IPTG and X-Gal. High target-gene expression is enabled by the presence of the lac promoter on both the pHSG396 and pHSG398 vectors.
Both pHSG 396 and pHSG 398 can be analyzed via dideoxy DNA sequencing using an M13 primer, and large recombinant chloramphenicol resistant pUC vectors may be analyzed using the Deletion Kit for Kilo-Sequencing.
Overview
- Form: 10 mM Tris-HCl (pH 8.0), 1 mM EDTA
- Concentration: 250–1,000 µg/ml
Applications
- lac promoter-enabled target gene cloning and expression
- DNA sequencing using an M13 primer
- Long DNA sequencing using Takara's Deletion Kit for Kilo-Sequencing
Source
pUC18 vector
Purity
- >70% double-stranded, covalently closed circular form (RF 1) DNA
- Multiple cloning site sequence-verified via dideoxy DNA sequencing
Storage
–20°C
GenBank
*Except for the MCS, pHSG396 and pHSG398 have the same sequence as pHSG399.
Chain length
- pHSG396: 2,238 bp
- pHSG398: 2,227 bp
Chloramphenicol-resistant pUC vectors pHSG396 and pHSG398
Schematic diagram and MCS sequence of the chloramphenicol resistant pUC vectors pHSG396 and pHSG398. Both vectors contain the chloramphenicol resistance gene, the pMB1-derived origin of replication (ori), and the beta-galactosidase coding gene lacZ. These chloramphenicol-resistant pUC vectors also contain a pUC18-derived multiple cloning site (MCS) within the lacZ gene, enabling recombinant clones to be verified through culture plates containing IPTG and X-Gal. The MCS in the two vectors is reversed.
References
Takeshita, S., Sato, M., Toba, M. & Masahashi, W. High-copy-number and low-copy-number plasmid vectors for lacZα-complementation and chloramphenicol-or kanamycin-resistance selection. Gene 61, 63-74 (1987).
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Source
pUC18 vector
Purity
- >70% double-stranded, covalently closed circular form (RF 1) DNA
- Multiple cloning site sequence-verified via dideoxy DNA sequencing
Storage
–20°C
GenBank
*Except for the MCS, pHSG396 and pHSG398 have the same sequence as pHSG399.
Chain length
- pHSG396: 2,238 bp
- pHSG398: 2,227 bp
Chloramphenicol-resistant pUC vectors pHSG396 and pHSG398
Schematic diagram and MCS sequence of the chloramphenicol resistant pUC vectors pHSG396 and pHSG398. Both vectors contain the chloramphenicol resistance gene, the pMB1-derived origin of replication (ori), and the beta-galactosidase coding gene lacZ. These chloramphenicol-resistant pUC vectors also contain a pUC18-derived multiple cloning site (MCS) within the lacZ gene, enabling recombinant clones to be verified through culture plates containing IPTG and X-Gal. The MCS in the two vectors is reversed.
