Cellartis Power Primary HEP Medium is specially formulated to enable the long-term maintenance—cells are viable and maintain functionality for four weeks—of cryopreserved, plateable human primary hepatocytes in conventional 2D cultures. This medium further simplifies culturing of hepatocytes with a weekend-free feeding schedule.
Overview
Extended culture time—keeps primary hepatocytes viable and metabolically active for four weeks in culture as measured by CYP activities, albumin secretion, and CYP induction capabilities
Long-term compound incubation—supports incubations of up to 10 days without medium change, enabling intrinsic clearance studies of low clearance compounds
Flexible format—delivered frozen; may be aliquoted and refrozen
Convenient workflow—allows a weekend-free feeding schedule [with media changes every other day (Mon, Wed, Fri) instead of daily] and eliminates complicated overlays or sandwich cultures
Customformulations—we offer customization of this product to better serve your research needs.
Primary hepatocytes cultured in Cellartis Power Primary HEP Medium
Cryoplateable human primary hepatocytes from three different vendors (1, 2, and 3) cultured in Cellartis Power Primary HEP Medium. Cells were cultured as described and images were taken 1 (Panel A), 7 (Panel B), 14 (Panel C), 21 (Panel D) and 28 (Panel E) days after thawing. For all images, the scale bar is 100 μm.
Cellartis Power Primary HEP Medium is specially formulated to enable the long-term maintenance—cells are viable and maintain functionality for four weeks—of cryopreserved, plateable human primary hepatocytes in conventional 2D cultures. This medium further simplifies culturing of hepatocytes with a weekend-free feeding schedule.
Overview
Extended culture time—keeps primary hepatocytes viable and metabolically active for four weeks in culture as measured by CYP activities, albumin secretion, and CYP induction capabilities
Long-term compound incubation—supports incubations of up to 10 days without medium change, enabling intrinsic clearance studies of low clearance compounds
Flexible format—delivered frozen; may be aliquoted and refrozen
Convenient workflow—allows a weekend-free feeding schedule [with media changes every other day (Mon, Wed, Fri) instead of daily] and eliminates complicated overlays or sandwich cultures
Customformulations—we offer customization of this product to better serve your research needs.
Primary hepatocytes cultured in Cellartis Power Primary HEP Medium
Cryoplateable human primary hepatocytes from three different vendors (1, 2, and 3) cultured in Cellartis Power Primary HEP Medium. Cells were cultured as described and images were taken 1 (Panel A), 7 (Panel B), 14 (Panel C), 21 (Panel D) and 28 (Panel E) days after thawing. For all images, the scale bar is 100 μm.