Cellartis iPS Cell to Hepatocyte Differentiation System
Cellartis iPS Cell to Hepatocyte Differentiation System
Brand: Takara Bio.
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Cellartis iPS Cell to Hepatocyte Differentiation System
Cellartis Hepatocyte Maintenance Medium are used for prolonged maintenance of hepatocyte cells in 2D culture, derived using Cellartis Hepatocyte Differentiation Kit (Cat. No. Y30050) or Cellartis iPS Cell to Hepatocyte Differentiation System (Cat. No. Y30055).
The Cellartis iPS Cell to Hepatocyte Differentiation System is a complete system for differentiation of human induced pluripotent stem (iPS) cells to hepatocytes via definitive endoderm (DE). This kit includes the Cellartis DEF-CS Culture System for expansion of undifferentiated iPS cells, the Cellartis Definitive Endoderm Differentiation Kit for differentiation to DE cells, and the Cellartis Hepatocyte Differentiation Kit for subsequent differentiation to hepatocytes.
Overview
- Highly reproducible, robust system—the exact same protocol has been shown to work across 25 different iPS cell lines, so there's no need to optimize for your lines
- Ideal for drug metabolism and safety studies—consistently generate panels of >90% pure, functional, hiPS cell-derived hepatocytes with diverse genetic backgrounds
- Customized starting materials—start with any disease-relevant iPS cell lines and create accurate liver disease models
- Ready for personalized medicine—make patient-specific, disease-specific cells for therapeutic applications
- Extended experimental window—hepatocytes can be used for a minimum of 11 days for functional tests
Components
- Cellartis DEF-CS 100 Culture System (Cat. # Y30020, not sold separately)
- Cellartis Definitive Endoderm Differentiation Kit (Cat. # Y30030, not sold separately)
- Cellartis Hepatocyte Differentiation Kit (Cat. # Y30050)
Immunocytochemistry analysis of hepatocyte differentiation
Immunocytochemistry analysis of hepatocyte differentiation. hiPS cells were differentiated into functional hepatocytes using the Cellartis iPS Cell to Hepatocyte Differentiation System. Hepatocytes were immunostained to detect early hepatic markers HNF4α (red, nuclear) and CK18 (green) on days 14 and 21. As the hepatocytes matured, expression of liver-specific markers CYP3A (red, cytoplasmic) and Albumin (green) increased as seen on day 28, as expected. Cell nuclei were stained with DAPI (blue).
Asplund, A. et al. One Standardized Differentiation Procedure Robustly Generates Homogenous Hepatocyte Cultures Displaying Metabolic Diversity from a Large Panel of Human Pluripotent Stem Cells. Stem Cell Rev. Reports 12, 90–104 (2016).
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Immunocytochemistry analysis of hepatocyte differentiation
Immunocytochemistry analysis of hepatocyte differentiation. hiPS cells were differentiated into functional hepatocytes using the Cellartis iPS Cell to Hepatocyte Differentiation System. Hepatocytes were immunostained to detect early hepatic markers HNF4α (red, nuclear) and CK18 (green) on days 14 and 21. As the hepatocytes matured, expression of liver-specific markers CYP3A (red, cytoplasmic) and Albumin (green) increased as seen on day 28, as expected. Cell nuclei were stained with DAPI (blue).
