Capturem Trypsin 96-Well Plate (Mass Spectrometry Grade)
Capturem Trypsin 96-Well Plate (Mass Spectrometry Grade)
Brand: Takara Bio.
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Capturem Trypsin 96-Well Plate (Mass Spectrometry Grade)
Capturem Trypsin 96-Well Plate (Mass Spectrometry Grade) enables rapid and efficient digestion of proteins, including antibodies, at room temperature. This product uses our Capturem technology with membrane-immobilized trypsin from porcine pancreas that has been modified by reductive methylation to increase its stability and resistance to autolytic digestion. Capturem Trypsin-mediated digestion of protein samples takes less than 4 minutes. This kit includes 50 ml Capturem Trypsin Activation Buffer.
Overview
- Digest protein samples in less than four minutes instead of overnight
- Generate fewer autolyzed trypsin fragments and protein modifications
- Get an uninterrupted proteomics analysis workflow
- Get more effective peptide and protein identification in MS analysis
- Process up to 80 µg of protein or 25 µg of antibody on each well or column
*All the mass spectrometry grade products have been manufactured with our new porcine dimethylated trypsin to increase stability and prevent autolysis more efficiently. Our older product, Capturem Trypsin Miniprep Kit, Cat. # 635722, manufactured with bovine trypsin will be discontinued soon.
Applications
- Protein characterization
- Proteomic analysis
- Mass spectrometry sample preparation
- Antibody characterization
Capturem Trypsin digestion workflow.
Capturem Trypsin digestion workflow. After initial activation of the columns with the included Capturem Trypsin Activation Buffer, the sample solution (50–800 µl) containing the native or denatured sample is passed through the spin column via two-minute centrifugation. Digestion by trypsin occurs on the column, and subsequent peptide fragments are eluted in a second two-minute centrifugation.
HPLC profiles from tryptic spin digest of 80 µg apomyoglobin under native conditions.
HPLC profiles from 80 µg of apomyoglobin digested using Capturem Trypsin under native conditions. Tryptic spin digests of 80 μg apomyoglobin under native conditions gives reproducible HPLC profiles.
Quantitation of SILuLite digested with Capturem trypsin.
Quantitation of SILuLite digested with Capturem trypsin. Tryptic spin digest of SILuLite yields surrogate peptides detectable at ng-input levels (1, 10, 50 ng). AUCs of specific peptides give a linear correlation.
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Capturem Trypsin digestion workflow.
Capturem Trypsin digestion workflow. After initial activation of the columns with the included Capturem Trypsin Activation Buffer, the sample solution (50–800 µl) containing the native or denatured sample is passed through the spin column via two-minute centrifugation. Digestion by trypsin occurs on the column, and subsequent peptide fragments are eluted in a second two-minute centrifugation.
HPLC profiles from tryptic spin digest of 80 µg apomyoglobin under native conditions.
HPLC profiles from 80 µg of apomyoglobin digested using Capturem Trypsin under native conditions. Tryptic spin digests of 80 μg apomyoglobin under native conditions gives reproducible HPLC profiles.
Quantitation of SILuLite digested with Capturem trypsin.
Quantitation of SILuLite digested with Capturem trypsin. Tryptic spin digest of SILuLite yields surrogate peptides detectable at ng-input levels (1, 10, 50 ng). AUCs of specific peptides give a linear correlation.
