Capturem Protein G Miniprep Columns
Capturem Protein G Miniprep Columns
Brand: Takara Bio.
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Capturem Protein G Miniprep Columns
Rapid Protein G-based antibody purification
Capturem Protein G Miniprep Columns consist of single-use, disposable columns for simple, rapid purification of antibodies that yield 50–100 μg of purified antibody per column, depending upon the sample type, species, and antibody isotype. These miniprep columns are suitable for purification of antibodies from animal sera, ascites fluid, cell culture media, and other sources. Each column can hold up to 850 µl of sample and requires a minimum elution volume of 200 µl.
Overview
- Fast and convenient: room-temperature protocol, complete in under 15 minutes, without incubation
- High purity: small column bed volumes trap fewer contaminants and allow large-volume washes
- High quality: short residence times reduce the possibility of aggregation or loss of activity
- Flexible: purify a wide range of antibodies
Applications
- Antibody purification
- Immunoprecipitation
Capturem Protein A- or G-functionalized membranes
Antibody purification workflow for Capturem Protein A or G 96-well plates. Each well can be loaded with up to 850 µl of a diluted sample (antibody sample diluted from 1:1 to 1:20 with buffer). Following binding, the membrane is then washed and the bound antibiody eluted with the appropriate buffers. Note: Protocol time varies for each format. Check user manuals for specifics on protocol times.
Capturem spin columns and filtration devices
Available Capturem spin column and filtration device formats. Pictured from left to right: nanoprep, miniprep, 96-well, 24-well, maxiprep, and large-volume Capturem formats. Formats available vary for the different functionalized membranes. Check product pages for list of available formats.
Load volumes and approximate yields for available Capturem formats by chemistry
Load volumes and yields of Capturem purification formats.
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Capturem Protein A- or G-functionalized membranes
Antibody purification workflow for Capturem Protein A or G 96-well plates. Each well can be loaded with up to 850 µl of a diluted sample (antibody sample diluted from 1:1 to 1:20 with buffer). Following binding, the membrane is then washed and the bound antibiody eluted with the appropriate buffers. Note: Protocol time varies for each format. Check user manuals for specifics on protocol times.
Capturem spin columns and filtration devices
Available Capturem spin column and filtration device formats. Pictured from left to right: nanoprep, miniprep, 96-well, 24-well, maxiprep, and large-volume Capturem formats. Formats available vary for the different functionalized membranes. Check product pages for list of available formats.
Load volumes and approximate yields for available Capturem formats by chemistry
Load volumes and yields of Capturem purification formats.
