Capturem Protein A Maxiprep Columns
Capturem Protein A Maxiprep Columns
Brand: Takara Bio.
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Capturem Protein A Maxiprep Columns
Rapid Capturem Protein A-based antibody purification
Capturem Protein A Maxiprep is a single-use disposable column for simple, rapid purification of antibodies that yields up to 2.5 mg of purified antibody per column, depending upon antibody isotype and species. This maxiprep column is suitable for purification of antibodies from animal sera, ascites fluid, cell culture media and other sources. Each column can hold up to 23 ml of sample and requires a minimum elution volume of 1.5 ml.
Overview
- No-waiting workflow: 5–15-minute, room-temperature protocol
- High purity: small column bed volumes trap fewer contaminants and allow large-volume washes
- High concentration: extremely small elution volumes yield highly concentrated antibodies
- High quality: short residence times reduce the possibility of aggregation or loss of activity
- Versatile: purify a wide range of antibodies
Applications
- Antibody purification
- Antibody screening
- Immunoprecipitation and co-immunoprecipitation (For a complete IP solution, see Capturem IP & Co-IP Kit)
- Approximate yields for Capturem Protein A formats
Capturem Protein A- or G-functionalized membranes
Antibody purification workflow for Capturem Protein A or G 96-well plates. Each well can be loaded with up to 850 µl of a diluted sample (antibody sample diluted from 1:1 to 1:20 with buffer). Following binding, the membrane is then washed and the bound antibiody eluted with the appropriate buffers. Note: Protocol time varies for each format. Check user manuals for specifics on protocol times.
Capturem spin columns and filtration devices
Available Capturem spin column and filtration device formats. Pictured from left to right: nanoprep, miniprep, 96-well, 24-well, maxiprep, and large-volume Capturem formats. Formats available vary for the different functionalized membranes. Check product pages for list of available formats.
Load volumes and approximate yields for available Capturem formats by chemistry
Load volumes and yields of Capturem purification formats.
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Capturem Protein A- or G-functionalized membranes
Antibody purification workflow for Capturem Protein A or G 96-well plates. Each well can be loaded with up to 850 µl of a diluted sample (antibody sample diluted from 1:1 to 1:20 with buffer). Following binding, the membrane is then washed and the bound antibiody eluted with the appropriate buffers. Note: Protocol time varies for each format. Check user manuals for specifics on protocol times.
Capturem spin columns and filtration devices
Available Capturem spin column and filtration device formats. Pictured from left to right: nanoprep, miniprep, 96-well, 24-well, maxiprep, and large-volume Capturem formats. Formats available vary for the different functionalized membranes. Check product pages for list of available formats.
Load volumes and approximate yields for available Capturem formats by chemistry
Load volumes and yields of Capturem purification formats.
