Capturem IP & Co-IP Kit
Capturem IP & Co-IP Kit
Brand: Takara Bio.
In stock
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Capturem IP & Co-IP Kit
Simple and rapid capture of protein-protein complexes from whole cell extracts.
Rapid, Protein A-based IP—Capturem IP & Co-IP Kit
The Capturem IP & Co-IP Kit is designed for the simple and rapid capture of protein-protein complexes from whole cell extracts. The kit contains specially designed Protein A spin columns, using our novel Capturem technology for efficient capture of antibody-bound target protein complexes. The included buffer set provides ease of use and rapid isolation of immunoprecipitated protein complexes, allowing faster downstream analysis.
Overview
- Simple, ultra-fast workflow: incubate IP antibody with sample for 10 minutes then purify at room temperature in 5 minutes
- Complete solution: includes Capturem Protein A spin columns and immunoprecipitation buffers optimized for IP and Co-IP experiments
- High concentration: extremely small elution volumes yield highly concentrated complexes
- High quality: short residence times reduce the possibility of aggregation, complex dissociation, or loss of activity
- Versatility: compatible with a wide range of IP buffers and conditions
- Single-use: novel membrane technology assembled into disposable spin columns reduces the risk of contamination and carryover
Applications
- Immunoprecipitation
- Co-immunoprecipitation
- Investigation of protein complexes
- Antigen screening for antibody discovery and engineering workflows
Comparison of Capturem IP & Co-IP Kit workflow to that of a leading competitor
Comparison of Capturem IP & Co-IP Kit workflow to that of a leading competitor.
Capturem IP & Co-IP Kit workflow
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Capturem IP & Co-IP Kit workflow. Each mini spin column can be loaded with up to 800 μl of sample containing the antibody-antigen complex. Antibodies are first bound to equilibrated membrane, followed by washing with 100 μl of wash buffer, and elution with 30–50 μl of elution buffer. Each step is followed by spinning the tube for 1 min at 1,000g. This entire purification is complete in ~15 min.
Capturem Protein A technology
Capturem Protein A technology. Each Capturem Protein A spin column contains a high-capacity Protein A membrane with increased surface area, providing much higher antibody binding capabilities per ml of membrane than per ml of resin.
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Comparison of Capturem IP & Co-IP Kit workflow to that of a leading competitor
Comparison of Capturem IP & Co-IP Kit workflow to that of a leading competitor.
Capturem IP & Co-IP Kit workflow
Capturem IP & Co-IP Kit workflow. Each mini spin column can be loaded with up to 800 μl of sample containing the antibody-antigen complex. Antibodies are first bound to equilibrated membrane, followed by washing with 100 μl of wash buffer, and elution with 30–50 μl of elution buffer. Each step is followed by spinning the tube for 1 min at 1,000g. This entire purification is complete in ~15 min.
Capturem Protein A technology
Capturem Protein A technology. Each Capturem Protein A spin column contains a high-capacity Protein A membrane with increased surface area, providing much higher antibody binding capabilities per ml of membrane than per ml of resin.
