Capturem His-Tagged Purification Maxiprep Kit
Capturem His-Tagged Purification Maxiprep Kit
Brand: Takara Bio.
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Capturem His-Tagged Purification Maxiprep Kit
Capturem high-capacity membranes
The Capturem His-Tagged Purification Maxiprep Kit provides single-use disposable membrane spin columns for simple, rapid purification of his-tagged proteins in up to 25 ml of clarified lysate. The columns are suitable for use under native or denaturing conditions, and in the presence of additives such as DTT (up to 10 mM), βME (up to 30 mM), TCEP (up to 5 mM), EDTA (up to 10 mM), or glycerol.
Overview
- No-waiting workflow—5-minute, room-temperature protocol
- High purity and yield—small column bed volumes trap fewer contaminants and allow large-volume washes
- Compatible with wide range of conditions—native and denaturing conditions; with common additives (e.g., EDTA, DTT, BME, glycerol, TCEP, etc.; see compatibility table)
- Lysis buffer compatibility—xTractor Buffer; buffers from other vendors
- Different cell systems—easy purification from mammalian and bacterial cells
Applications
- Biochemical/enzymatic assays
- Pull-down or other protein interaction assays
- Confirmation screening
- Protein/ligand affinity analysis
- Crystallography or other structural analyses
- Antibody production
- Toxicology studies
- Protein transfer to live cells
High yield and activity of his-tagged Metridia luciferase purified using the Capturem His-Tagged Purification Maxiprep Kit
High yield and activity of his-tagged Metridia luciferase purified using the Capturem His-Tagged Purification Maxiprep Kit. Panel A. Confirmation of Metridia luciferase expression in 293T cells. The pEF1a-Metluc-6xHis-IRES2-DsRed-Express2 expression vector, which coexpresses 6xhis-tagged Metridia luciferase with a red fluorescent reporter, was constructed using In-Fusion Cloning and the pEF1alpha-IRES-DsRed-Express2 Vector, and transfected into 293T cells using Xfect Transfection Reagent. The DsRed-Express2 reporter was then used to monitor transfection efficiency. Panel B. Yield and activity of Metridia luciferase, pre- and post-purification. Luciferase activity was measured at various steps in the purification of culture supernatant prepared from 293T cells expressing his-tagged Metridia luciferase.
Capturem Protein A- or G-functionalized membranes
Antibody purification workflow for Capturem Protein A or G 96-well plates. Each well can be loaded with up to 850 µl of a diluted sample (antibody sample diluted from 1:1 to 1:20 with buffer). Following binding, the membrane is then washed and the bound antibiody eluted with the appropriate buffers. Note: Protocol time varies for each format. Check user manuals for specifics on protocol times.
Capturem spin columns and filtration devices
Available Capturem spin column and filtration device formats. Pictured from left to right: nanoprep, miniprep, 96-well, 24-well, maxiprep, and large-volume Capturem formats. Formats available vary for the different functionalized membranes. Check product pages for list of available formats.
Load volumes and approximate yields for available Capturem formats by chemistry
Load volumes and yields of Capturem purification formats.
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High yield and activity of his-tagged Metridia luciferase purified using the Capturem His-Tagged Purification Maxiprep Kit
High yield and activity of his-tagged Metridia luciferase purified using the Capturem His-Tagged Purification Maxiprep Kit. Panel A. Confirmation of Metridia luciferase expression in 293T cells. The pEF1a-Metluc-6xHis-IRES2-DsRed-Express2 expression vector, which coexpresses 6xhis-tagged Metridia luciferase with a red fluorescent reporter, was constructed using In-Fusion Cloning and the pEF1alpha-IRES-DsRed-Express2 Vector, and transfected into 293T cells using Xfect Transfection Reagent. The DsRed-Express2 reporter was then used to monitor transfection efficiency. Panel B. Yield and activity of Metridia luciferase, pre- and post-purification. Luciferase activity was measured at various steps in the purification of culture supernatant prepared from 293T cells expressing his-tagged Metridia luciferase.
Capturem Protein A- or G-functionalized membranes
Antibody purification workflow for Capturem Protein A or G 96-well plates. Each well can be loaded with up to 850 µl of a diluted sample (antibody sample diluted from 1:1 to 1:20 with buffer). Following binding, the membrane is then washed and the bound antibiody eluted with the appropriate buffers. Note: Protocol time varies for each format. Check user manuals for specifics on protocol times.
Capturem spin columns and filtration devices
Available Capturem spin column and filtration device formats. Pictured from left to right: nanoprep, miniprep, 96-well, 24-well, maxiprep, and large-volume Capturem formats. Formats available vary for the different functionalized membranes. Check product pages for list of available formats.
Load volumes and approximate yields for available Capturem formats by chemistry
Load volumes and yields of Capturem purification formats.
