Capturem His-Tagged Purification 96-Well Plate
Capturem His-Tagged Purification 96-Well Plate
Brand: Takara Bio.
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Capturem His-Tagged Purification 96-Well Plate
Capturem high-capacity membranes
Capturem His-Tagged Purification 96 is a single-use, disposable 96-well plate for simple, rapid purification of his-tagged proteins from up to 1 ml of clarified lysate per well. This plate is suitable for use under native or denaturing conditions, in the presence of additives such as DTT (up to 10 mM), βME (up to 30 mM), TCEP (up to 5 mM), EDTA (up to 10 mM), or glycerol. Capturem His-Tagged Purification 96 is compatible with automated liquid handlers equipped with vacuum filtration or centrifuges equipped with appropriate plate holders.
Overview
- No-waiting workflow—5-minute, room-temperature protocol
- High purity and yield—small column bed volumes trap fewer contaminants and allow large-volume washes
- Compatible with wide range of conditions—native and denaturing conditions; with common additives (e.g., EDTA, DTT, BME, glycerol, TCEP, etc.; see compatibility table)
- Lysis buffer compatibility—xTractor Buffer; buffers from other vendors
- Different cell systems—easy purification from mammalian and bacterial cells
Applications
- Biochemical/enzymatic assays
- Pull-down or other protein interaction assays
- Confirmation screening
- Protein/ligand affinity analysis
- Crystallography or other structural analyses
- Antibody production
- Toxicology studies
- Protein transfer to live cells
Capturem Protein A- or G-functionalized membranes
Antibody purification workflow for Capturem Protein A or G 96-well plates. Each well can be loaded with up to 850 µl of a diluted sample (antibody sample diluted from 1:1 to 1:20 with buffer). Following binding, the membrane is then washed and the bound antibiody eluted with the appropriate buffers. Note: Protocol time varies for each format. Check user manuals for specifics on protocol times.
Capturem spin columns and filtration devices
Available Capturem spin column and filtration device formats. Pictured from left to right: nanoprep, miniprep, 96-well, 24-well, maxiprep, and large-volume Capturem formats. Formats available vary for the different functionalized membranes. Check product pages for list of available formats.
Load volumes and approximate yields for available Capturem formats by chemistry
Load volumes and yields of Capturem purification formats.
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Capturem Protein A- or G-functionalized membranes
Antibody purification workflow for Capturem Protein A or G 96-well plates. Each well can be loaded with up to 850 µl of a diluted sample (antibody sample diluted from 1:1 to 1:20 with buffer). Following binding, the membrane is then washed and the bound antibiody eluted with the appropriate buffers. Note: Protocol time varies for each format. Check user manuals for specifics on protocol times.
Capturem spin columns and filtration devices
Available Capturem spin column and filtration device formats. Pictured from left to right: nanoprep, miniprep, 96-well, 24-well, maxiprep, and large-volume Capturem formats. Formats available vary for the different functionalized membranes. Check product pages for list of available formats.
Load volumes and approximate yields for available Capturem formats by chemistry
Load volumes and yields of Capturem purification formats.
