Capturem His-tagged 24-Well Plates are 24-well plates that provide rapid, high-quality, small-scale purification of his-tagged proteins. These plates combine Ni-NTA chemistry with novel Capturem technology to provide high-capacity, membrane-based affinity purification. Typical yields for his-tagged proteins are up to 800 µg per well with a 4.5-ml sample capacity per well.
High purity and yield—small column bed volumes trap fewer contaminants and allow large-volume washes
Compatible with wide range of conditions—native and denaturing conditions; with common additives (e.g., EDTA, DTT, BME, glycerol, TCEP, etc.; see compatibility table)
Lysis buffer compatibility—xTractor Buffer; buffers from other vendors
Different cell systems—easy purification from mammalian and bacterial cells
Antibody purification workflow for Capturem Protein A or G 96-well plates. Each well can be loaded with up to 850 µl of a diluted sample (antibody sample diluted from 1:1 to 1:20 with buffer). Following binding, the membrane is then washed and the bound antibiody eluted with the appropriate buffers. Note: Protocol time varies for each format. Check user manuals for specifics on protocol times.
Capturem spin columns and filtration devices
Available Capturem spin column and filtration device formats. Pictured from left to right: nanoprep, miniprep, 96-well, 24-well, maxiprep, and large-volume Capturem formats. Formats available vary for the different functionalized membranes. Check product pages for list of available formats.
Load volumes and approximate yields for available Capturem formats by chemistry
Load volumes and yields of Capturem purification formats.
Capturem His-tagged 24-Well Plates are 24-well plates that provide rapid, high-quality, small-scale purification of his-tagged proteins. These plates combine Ni-NTA chemistry with novel Capturem technology to provide high-capacity, membrane-based affinity purification. Typical yields for his-tagged proteins are up to 800 µg per well with a 4.5-ml sample capacity per well.
High purity and yield—small column bed volumes trap fewer contaminants and allow large-volume washes
Compatible with wide range of conditions—native and denaturing conditions; with common additives (e.g., EDTA, DTT, BME, glycerol, TCEP, etc.; see compatibility table)
Lysis buffer compatibility—xTractor Buffer; buffers from other vendors
Different cell systems—easy purification from mammalian and bacterial cells
Antibody purification workflow for Capturem Protein A or G 96-well plates. Each well can be loaded with up to 850 µl of a diluted sample (antibody sample diluted from 1:1 to 1:20 with buffer). Following binding, the membrane is then washed and the bound antibiody eluted with the appropriate buffers. Note: Protocol time varies for each format. Check user manuals for specifics on protocol times.
Capturem spin columns and filtration devices
Available Capturem spin column and filtration device formats. Pictured from left to right: nanoprep, miniprep, 96-well, 24-well, maxiprep, and large-volume Capturem formats. Formats available vary for the different functionalized membranes. Check product pages for list of available formats.
Load volumes and approximate yields for available Capturem formats by chemistry
Load volumes and yields of Capturem purification formats.