Capturem Extracellular Vesicle Isolation Kits
Capturem Extracellular Vesicle Isolation Kits
Brand: Takara Bio.
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Capturem Extracellular Vesicle Isolation Kits
The Capturem Extracellular Vesicle Isolation Kit provides a complete solution for the simple and rapid isolation of extracellular vesicles, like exosomes, from plasma and other small volumes of biological fluids. The kit includes 20 single-use, disposable pre-clearing columns; 20 single-use, disposable isolation columns; as well as equilibrium, wash, and elution buffers. Extracellular vesicles are isolated using specially designed Capturem spin columns containing a lectin-based binding compound which is not antibody-based.
Overview
- Consistent, rapid isolation of highly pure, concentrated EVs from up to 850 µl of biofluid, in <30 minutes
- EVs express exosome protein markers, but not nonexosomal contaminant proteins such as calnexin or albumin
- Sufficient EV yield (up to 1010) for downstream RT-qPCR or NGS analysis of exosomal RNA
- Purified EVs display classical morphology, as shown by TEM
Capturem Extracellular Vesicle Isolation Kit workflow
Rapid workflow for extracellular vesicle (EV) purification with the Capturem Extracellular Vesicle Isolation Kit. In this workflow, the supernatant is collected following pre-clearing and filtration steps that remove cellular debris and non-EV proteins, respectively. EVs are then bound to the equilibrated membrane and washed and eluted with appropriate buffers. Each step is followed by spinning the tube using a benchtop centrifuge for just 2 minutes. *Filtration time will vary depending on sample type and volume.
Jeppesen, D. K., et al. Comparative analysis of discrete exosome fractions obtained by differential centrifugation. J. Extracell. Vesicles 3, 25011 (2014).
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Capturem Extracellular Vesicle Isolation Kit workflow
Rapid workflow for extracellular vesicle (EV) purification with the Capturem Extracellular Vesicle Isolation Kit. In this workflow, the supernatant is collected following pre-clearing and filtration steps that remove cellular debris and non-EV proteins, respectively. EVs are then bound to the equilibrated membrane and washed and eluted with appropriate buffers. Each step is followed by spinning the tube using a benchtop centrifuge for just 2 minutes. *Filtration time will vary depending on sample type and volume.
