Brevibacillus Competent Cells are host cells used for transformation with Brevibacillus expression vectors such as pNY326 DNA (Cat. #HB111), pNCMO2 DNA (Cat. #HB112), and pNC-HisT DNA (Cat. #HB121). Brevibacillus Competent Cells are prepared for transformation using the New Tris-PEG (NTP) method for chemical transformation of B. choshinensis strain SP3, which can provide a transformation efficiency that is approximately equal to that obtained with electroporation. The NTP method also enables direct transformation of a DNA ligation solution without DNA purification.
Efficient production of secreted or intracellular recombinant target proteins
Negligible amounts of extracellular protease—products remain intact in culture medium
Brevibacillus Competent Cells are host cells used for transformation with Brevibacillus expression vectors such as pNY326 DNA (Cat. #HB111), pNCMO2 DNA (Cat. #HB112), and pNC-HisT DNA (Cat. #HB121). Brevibacillus Competent Cells are prepared for transformation using the New Tris-PEG (NTP) method for chemical transformation of B. choshinensis strain SP3, which can provide a transformation efficiency that is approximately equal to that obtained with electroporation. The NTP method also enables direct transformation of a DNA ligation solution without DNA purification.
Efficient production of secreted or intracellular recombinant target proteins
Negligible amounts of extracellular protease—products remain intact in culture medium