Ligation-recutting test: ligation efficiency of DNA fragments with cohesive end generated by this enzyme is lower than general 3-base protruding ends. Therefore, more efficient ligation can be achieved by using the reaction conditions for blunt-end ligation
Each lot undergoes stringent quality testing, including: overdigestion, genome DNA analysis, ligation-recutting, and pKF3 Cloning
Ligation-recutting test: ligation efficiency of DNA fragments with cohesive end generated by this enzyme is lower than general 3-base protruding ends. Therefore, more efficient ligation can be achieved by using the reaction conditions for blunt-end ligation
Each lot undergoes stringent quality testing, including: overdigestion, genome DNA analysis, ligation-recutting, and pKF3 Cloning
