Genomic DNA analysis: Escherichia coli genomic DNA
Ligation-recutting test: Ligation efficiency of DNA fragments with cohesive end generated by this enzyme is lower than general 4-base protruding ends. Therefore, more efficient ligation can be achieved by using the reaction conditions for blunt end ligation.
Star activity: cleavage at alternative site(s) occurs in the presence of Mn2+, DMSO, or at high ionic strength
Each lot undergoes stringent quality testing including overdigestion, genome DNA analysis, ligation-recutting and pKF3 cloning
Genomic DNA analysis: Escherichia coli genomic DNA
Ligation-recutting test: Ligation efficiency of DNA fragments with cohesive end generated by this enzyme is lower than general 4-base protruding ends. Therefore, more efficient ligation can be achieved by using the reaction conditions for blunt end ligation.
Star activity: cleavage at alternative site(s) occurs in the presence of Mn2+, DMSO, or at high ionic strength
Each lot undergoes stringent quality testing including overdigestion, genome DNA analysis, ligation-recutting and pKF3 cloning
