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BglI
BglI restriction site:
GCCN NNN | NGGC
CGGN | NNN NCCG
Supplied buffer: basal
Effect of DNA methylation: enzyme activity is affected by CG methylase
Reaction temperature: 37°C
- Source: Bacillus globigii
- Concentration: 10 units/µl
- Substrate for unit definition: lambda DNA
- Genome DNA analysis: Staphylococcus aureus genome DNA
- Star activity: unrelated site may often be cut at low ionic strength
- Each lot undergoes stringent quality testing including overdigestion, genome DNA analysis, ligation-recutting and pKF3 cloning
BglI restriction site:
GCCN NNN | NGGC
CGGN | NNN NCCG
Supplied buffer: basal
Effect of DNA methylation: enzyme activity is affected by CG methylase
Reaction temperature: 37°C
- Source: Bacillus globigii
- Concentration: 10 units/µl
- Substrate for unit definition: lambda DNA
- Genome DNA analysis: Staphylococcus aureus genome DNA
- Star activity: unrelated site may often be cut at low ionic strength
- Each lot undergoes stringent quality testing including overdigestion, genome DNA analysis, ligation-recutting and pKF3 cloning
