Ligation-recutting test: After digestion with this enzyme, the ligation is inefficient under the influence of the structure of BcnI fragments ends (Janulaitis, A.A., personal information). For circularized ligation, overnight ligation using TaKaRa DNA Ligation Kit Ver. 2.1 or Mighty Mix is recommended.
Each lot undergoes stringent quality testing including overdigestion, genome DNA analysis, ligation-recutting and pKF3 cloning
Ligation-recutting test: After digestion with this enzyme, the ligation is inefficient under the influence of the structure of BcnI fragments ends (Janulaitis, A.A., personal information). For circularized ligation, overnight ligation using TaKaRa DNA Ligation Kit Ver. 2.1 or Mighty Mix is recommended.
Each lot undergoes stringent quality testing including overdigestion, genome DNA analysis, ligation-recutting and pKF3 cloning
