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BanII (HgiJII)
BanII (HgiJII) restriction site:
G(A/G)GC(T/C) | C
C | (T/C)CG(A/G)G
Supplied buffer: H
Effect of DNA methylation: enzyme activity is not affected by CG methylase
Reaction temperature: 37°C
- Source: Bacillus aneurinolyticus
- Concentration: 4–12 units/μl
- Substrate for unit definition: lambda DNA
- Star activity: unrelated site may be cut in the presence of high concentrations of glycerol, DMSO, or low ionic strength
- Each lot undergoes stringent quality testing including overdigestion, genome DNA analysis, ligation-recutting and pKF3 cloning
BanII (HgiJII) restriction site:
G(A/G)GC(T/C) | C
C | (T/C)CG(A/G)G
Supplied buffer: H
Effect of DNA methylation: enzyme activity is not affected by CG methylase
Reaction temperature: 37°C
- Source: Bacillus aneurinolyticus
- Concentration: 4–12 units/μl
- Substrate for unit definition: lambda DNA
- Star activity: unrelated site may be cut in the presence of high concentrations of glycerol, DMSO, or low ionic strength
- Each lot undergoes stringent quality testing including overdigestion, genome DNA analysis, ligation-recutting and pKF3 cloning
