BamHI

BamHI

Brand: Takara Bio.
In stock
SKU
BamHI
Grouped product items
Product Name Size
BamHI
SKU: 1010A
10000 Units
BamHI
SKU: 1010B
50000 Units
BamHI (High concentration)
SKU: 1010AH
10000 Units
BamHI (High concentration)
SKU: 1010BH
50000 Units
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BamHI
BamHI

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BamHI restriction site:

G | GATC C
C CTAG | G

Supplied buffer: K

Effect of DNA methylation: enzyme activity is not affected by CG methylase, dam methylase, or dcm methylase

Reaction temperature: 30°C

  • Source: Escherichia coli carrying the plasmid encoding BamHI gene
  • Concentration: 8–20 units/μl [High concentration: 30–60 units/μl]
  • Substrate for unit definition: lambda DNA
  • Star activity: unrelated site may often be cut in the presence of high concentration of glycerol, Mn2+, or DMSO, and at low ionic strength
  • Notice: this enzyme shows equivalent activity at 37°C and to 30°C, but the stability of the enzyme is lower in reaction mixtures at 37°C than that at 30°C
  • Each lot undergoes stringent quality testing including overdigestion, genome DNA analysis, ligation-recutting and pKF3 cloning
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BamHI restriction site:

G | GATC C
C CTAG | G

Supplied buffer: K

Effect of DNA methylation: enzyme activity is not affected by CG methylase, dam methylase, or dcm methylase

Reaction temperature: 30°C

  • Source: Escherichia coli carrying the plasmid encoding BamHI gene
  • Concentration: 8–20 units/μl [High concentration: 30–60 units/μl]
  • Substrate for unit definition: lambda DNA
  • Star activity: unrelated site may often be cut in the presence of high concentration of glycerol, Mn2+, or DMSO, and at low ionic strength
  • Notice: this enzyme shows equivalent activity at 37°C and to 30°C, but the stability of the enzyme is lower in reaction mixtures at 37°C than that at 30°C
  • Each lot undergoes stringent quality testing including overdigestion, genome DNA analysis, ligation-recutting and pKF3 cloning
Write Your Own Review
You're reviewing:BamHI
Your Rating