Cleavage of pBR322: the commercially available pBR322 is substantially undigested because the cytosine residues in the recognition site (TGGCCA) are methylated by Dcm methylase
Each lot undergoes stringent quality testing including overdigestion, genome DNA analysis, ligation-recutting and pKF3 cloning
Cleavage of pBR322: the commercially available pBR322 is substantially undigested because the cytosine residues in the recognition site (TGGCCA) are methylated by Dcm methylase
Each lot undergoes stringent quality testing including overdigestion, genome DNA analysis, ligation-recutting and pKF3 cloning
