BAL 31 Nuclease acts as an exonuclease, degrading double-stranded DNA and RNA from both 5'-phosphate and 3'-hydroxyl termini. This enzyme also possesses single-stranded DNA and RNA endonuclease activity and is capable of cleaving at DNA/RNA nicks and gaps. It requires Ca2+ as an essential cofactor for its exonuclease and endonuclease activities.
BAL 31 Nuclease is supplied in 20 mM Tris-HCl (pH 8.0), 100 mM NaCl, 5 mM CaCl2, 5 mM MgCl2, 1 mM EDTA and 50% glycerol.
Gray, H. B. et al. The extracellular nuclease from Alteromonas espejiana: an enzyme highly specific for nonduplex structure in nominally duplex DNA. Gene Amplif. Anal.2, 169–203 (1981).
Sambrook, J., Fritsch, E. F. & Maniatis, T. Molecular cloning : a laboratory manual. (Cold Spring Harbor Laboratory, 1989).
BAL 31 Nuclease acts as an exonuclease, degrading double-stranded DNA and RNA from both 5'-phosphate and 3'-hydroxyl termini. This enzyme also possesses single-stranded DNA and RNA endonuclease activity and is capable of cleaving at DNA/RNA nicks and gaps. It requires Ca2+ as an essential cofactor for its exonuclease and endonuclease activities.
BAL 31 Nuclease is supplied in 20 mM Tris-HCl (pH 8.0), 100 mM NaCl, 5 mM CaCl2, 5 mM MgCl2, 1 mM EDTA and 50% glycerol.
Gray, H. B. et al. The extracellular nuclease from Alteromonas espejiana: an enzyme highly specific for nonduplex structure in nominally duplex DNA. Gene Amplif. Anal.2, 169–203 (1981).
Sambrook, J., Fritsch, E. F. & Maniatis, T. Molecular cloning : a laboratory manual. (Cold Spring Harbor Laboratory, 1989).