B/B Homodimerizer
B/B Homodimerizer
Brand: Takara Bio.
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B/B Homodimerizer
The B/B Homodimerizer is a membrane-permeant ligand that causes homodimerization of proteins fused to dimerization domain B (DmrB)—provided in the iDimerize Inducible Homodimer System (Cat. No. 635068). NOTE: The B/B Homodimerizer is identical to the AP20187 ligand previously supplied in the ARGENT Regulated Homodimerization Kit from ARIAD Pharmaceuticals, Inc.
B/B Washout Ligand is a membrane-permeant ligand that dissociates protein interactions induced by the B/B Homodimerizer, which is provided in each of the iDimerize Inducible Homodimer Systems (Cat. Nos. 635068, 635072, 635084, and 635086). The B/B Washout Ligand dissociates these interactions with a T1/2 of ~10 minutes after adding it to target cells treated with B/B Homodimerizer.
Overview
- Previously available from ARIAD as the ARGENT Regulated Homodimerization Kit
- Rapid chemical induction of cell signaling pathways
- Activate any pathway or cellular event controlled by homo-oligomers (e.g., dimers, trimers, or tetramers)
- Established tool used by over 2,000 research groups, with over 400 scientific publications to date
- B/B Homodimerizer is identical to the AP20187 ligand
- B/B Washout Ligand reverses protein oligomers that were induced by the B/B Homodimerizer
- Plasmid and lentiviral (Lenti-X) vector formats are available
Applications
- Induce any event or pathway that is controlled or activated by protein-protein interactions
- Activate signaling pathways in real time
- Study transmembrane receptors
- Recruit signaling proteins to specific subcellular locations (e.g., the plasma membrane)
- Inducible homodimer technology has been used for functional studies of:
- Receptor and non-receptor tyrosine kinases
- Receptor and non-receptor serine/threonine kinases
- Non-kinase receptors
- Signaling proteases
- Adaptor proteins
- Cell adhesion and rolling
- DNA looping
- Activation of recombinase
- RNA splicing
- Protein splicing
- Glycosylation
Signal transduction example 2
Signal transduction example 2. Inducible mouse model for prostate cancer. iDimerize Inducible Homodimer technology was used to characterize the role of different FGF receptor subtypes (FGFR1 and FGFR2) in prostate cancer. Transgenic mice (which express conditionally active alleles of FGFR1 or FGFR2 in prostate tissue) were treated with B/B Homodimerizer and monitored for prostate cancer. FGFR1, but not FGFR2, was found to play a role in prostate cancer development [Freeman, K. W., et al. (2003) Cancer Res. 63(23):8256–8563].
Signal transduction example 1: Inducing a Programmed Cell Death Pathway (Inducible Apoptosis)
Signal transduction example 1: Inducing a Programmed Cell Death Pathway (Inducible Apoptosis). The Fas receptor (FasR) is a transmembrane protein located on the surface of cells that activates programmed cell death (apoptosis) when induced to trimerize by fas ligand (FasL) molecules located on the surface of adjacent cells (e.g., cytotoxic T cells). FasR-FasL binding plays an important role in the regulation of the immune system and cancer progression. This trimerization (and the resulting apoptotic signaling cascade) can be mimicked using the iDimerize Inducible Homodimer System: cells expressing a FasR-DmrB fusion protein can induce cell death on demand, simply by adding the B/B Homodimerizer. An in vivo model [the MaFIA mouse; Burnett, S. H. et al. (2004) J. Leukoc. Biol. 75(4):612–623] utilizes the Fas receptor to systematically and reversibly eliminate macrophages from transgenic mice.
Molecular weights of iDimerize ligands
Molecular weights of iDimerize ligands.
Burnett, S. H. et al. Conditional macrophage ablation in transgenic mice expressing a Fas-based suicide gene. J. Leukoc. Biol. 75, 612–623 (2004).
Freeman, K. W. et al. Inducible prostate intraepithelial neoplasia with reversible hyperplasia in conditional FGFR1-expressing mice. Cancer Res. 63, 8256–63 (2003).
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Signal transduction example 2
Signal transduction example 2. Inducible mouse model for prostate cancer. iDimerize Inducible Homodimer technology was used to characterize the role of different FGF receptor subtypes (FGFR1 and FGFR2) in prostate cancer. Transgenic mice (which express conditionally active alleles of FGFR1 or FGFR2 in prostate tissue) were treated with B/B Homodimerizer and monitored for prostate cancer. FGFR1, but not FGFR2, was found to play a role in prostate cancer development [Freeman, K. W., et al. (2003) Cancer Res. 63(23):8256–8563].
Signal transduction example 1: Inducing a Programmed Cell Death Pathway (Inducible Apoptosis)
Signal transduction example 1: Inducing a Programmed Cell Death Pathway (Inducible Apoptosis). The Fas receptor (FasR) is a transmembrane protein located on the surface of cells that activates programmed cell death (apoptosis) when induced to trimerize by fas ligand (FasL) molecules located on the surface of adjacent cells (e.g., cytotoxic T cells). FasR-FasL binding plays an important role in the regulation of the immune system and cancer progression. This trimerization (and the resulting apoptotic signaling cascade) can be mimicked using the iDimerize Inducible Homodimer System: cells expressing a FasR-DmrB fusion protein can induce cell death on demand, simply by adding the B/B Homodimerizer. An in vivo model [the MaFIA mouse; Burnett, S. H. et al. (2004) J. Leukoc. Biol. 75(4):612–623] utilizes the Fas receptor to systematically and reversibly eliminate macrophages from transgenic mice.
Molecular weights of iDimerize ligands
Molecular weights of iDimerize ligands.
