Aureobasidin A
Aureobasidin A (AbA) is a cyclic depsipeptide antibiotic, isolated from the filamentous fungus Aureobasidium pullulans R106, which is toxic to yeast at low concentrations (0.1–0.5 μg/ml). Sensitive species include Saccharomyces cerevisiae, Schizosaccharomyces pombe, Candida glabrata, Aspergillus nidulans and A. niger. AbA inhibits a yeast enzyme, inositol phosphorylceramide (IPC) synthase, whichis expressed from the AUR1 gene. Expression of a mutant gene, AUR1-C, in transformed yeast confers resistance to the drug.
Overview
- Stringent drug-selectable marker for yeast
- Aureobasidin A resistance is an ideal reporter for yeast two-hybrid studies
- Provides exceptional screening results with Matchmaker Gold Yeast One- and Two-Hybrid Systems
Applications
- Yeast two-hybrid studies
- Yeast one-hybrid studies
Aureobasidin A (AbA) is a cyclic depsipeptide antibiotic that inhibits a yeast enzyme, inositol phosphorylceramide (IPC) synthase, expressed from the AUR1 gene
Aureobasidin A (AbA) is a cyclic depsipeptide antibiotic that inhibits a yeast enzyme, inositol phosphorylceramide (IPC) synthase, expressed from the AUR1 gene. Expression of a mutant gene, AUR1-C, in transformed yeast confers resistance to the drug. It is this gene that is used in Matchmaker Gold systems as a reporter for protein interactions.
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Aureobasidin A (AbA) is a cyclic depsipeptide antibiotic that inhibits a yeast enzyme, inositol phosphorylceramide (IPC) synthase, expressed from the AUR1 gene
Aureobasidin A (AbA) is a cyclic depsipeptide antibiotic that inhibits a yeast enzyme, inositol phosphorylceramide (IPC) synthase, expressed from the AUR1 gene. Expression of a mutant gene, AUR1-C, in transformed yeast confers resistance to the drug. It is this gene that is used in Matchmaker Gold systems as a reporter for protein interactions.
